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| Biotinylated Human P-Selectin/CD62P Protein (LTP10958) |
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| LTP10958 |
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| 100ug |
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$789 In stock |
| Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. |
| Recombinant Biotinylated Human P-Selectin/CD62P Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Trp42-Ala771. |
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| P-Selectin/CD62P |
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| Human |
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| P16109 |
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| Trp42-Ala771 |
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| The protein has a predicted MW of 82.9 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-P-selectin Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human P-Selectin, His Tag with the EC50 of 0.25ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% saccharose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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