|
| Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein (LTP10514) |
|
| LTP10514 |
|
| 100ug |
|
|
$789 In stock |
| Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression. |
| Recombinant Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Met22-Arg734. |
|
| Semaphorin 4D/SEMA4D/CD100 |
|
| Human |
|
| Q92854-1 |
|
| Met22-Arg734 |
|
| The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Anti-Semaphorin 4D Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Semaphorin 4D, His Tag with the EC50 of 44.8ng/ml determined by ELISA. See testing image for detail. |
|
| C-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
