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| Biotinylated Human TPBG/5T4 Protein (LTP10704) |
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| LTP10704 |
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| 100ug |
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$789 In stock |
| The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs. |
| Recombinant Biotinylated Human TPBG/5T4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ser32-Ser355. |
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| TPBG/5T4 |
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| Human |
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| Q13641-1 |
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| Ser32-Ser355 |
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| The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-TPBG Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TPBG, His Tag with the EC50 of 43.3ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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