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| Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein (LTP10966) |
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| LTP10966 |
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| 100ug |
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$789 In stock |
| CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. |
| Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Asn19-Pro152. |
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| CD28 |
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| Human/Cynomolgus/Rhesus macaque |
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| P10747 |
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| Asn19-Pro152 |
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| The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-CD28 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA. See testing image for detail. |
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| N-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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