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| Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein (LTP10580) |
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| LTP10580 |
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| 100ug |
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$1013 In stock |
| GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-? and thus promoting secretion and activation of TGF-?. Integrin ?v?8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-? from the TGF-?&GARP complex. |
| Recombinant Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Ala18-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1). |
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| GARP&Latent TGF beta 1 Complex |
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| Mouse |
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| G3XA59(GARP)&P04202(Latent TGF beta 1) |
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| Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1) |
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| The protein has a predicted MW of 66.6 kDa (GARP)&44.2 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-76 kDa (GARP)&13 kDa&45-47 kDa (Latent TGF beta 1) based on the Tris-Bis PAGE result. |
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| Immobilized Mouse ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail. |
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| N-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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