| Biotinylated Mouse GFRAL/GFR alpha-like Protein (LTP10131) |
| LTP10131 |
| 100ug |
|
$900 In stock |
| GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. |
| Recombinant Biotinylated Mouse GFRAL/GFR alpha-like Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Gln20-Glu350. |
| GFRAL/GFR alpha-like |
| Mouse |
| Q6SJE0-1 |
| Gln20-Glu350 |
| The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on the Tris-Bis PAGE result. |
| Immobilized Biotinylated Mouse GFRAL, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. See testing image for detail. |
| N-His-Avi |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| ![Biotinylated Mouse GFRAL/GFR alpha-like Protein (LTP10131)](/peptide-product/images/protein-products/LTP10131/20220511190929.png) |