Biotinylated Mouse uPAR/PLAUR isoform 1 Protein (LTP10057)

Product Name
Biotinylated Mouse uPAR/PLAUR isoform 1 Protein (LTP10057)
Catalog Number
LTP10057
Quantity
100ug
Price
$789 In stock
Background
The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.
Description
Recombinant Biotinylated Mouse uPAR/PLAUR isoform 1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu24-Gly298.
Target
uPAR/PLAUR isoform 1
Species
Mouse
Gene/Protein ID
P35456-1
Exact Sequence
Leu24-Gly298
Molecular Weight
The protein has a predicted MW of 32.92 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human PLAU, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse uPAR isoform 1, His Tag with the EC50 of 2.57ug/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Mouse uPAR/PLAUR isoform 1 Protein (LTP10057)
  • 5 Units in Stock

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