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| Cleaved PARP (Asp214) Rabbit mAb |
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| LTA24810 |
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| 100ul |
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$750
In stock
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| Enables NAD+ ADP-ribosyltransferase activity; chromatin binding activity; and protein ADP-ribosylase activity. Involved in negative regulation of telomere maintenance via telomere lengthening; positive regulation of single strand break repair; and voluntary musculoskeletal movement. Acts upstream of or within several processes, including DNA metabolic process; behavioral response to cocaine; and protein poly-ADP-ribosylation. Located in cytoplasm; nucleolus; and nucleoplasm. Part of protein-containing complex. Is expressed in several structures, including Peyer's patch; brain; gut; immune system; and retina. Human ortholog(s) of this gene implicated in several diseases, including arthritis; bacterial meningitis; neurodegenerative disease (multiple); stomach carcinoma; and type 2 diabetes mellitus. Orthologous to human PARP1 (poly(ADP-ribose) polymerase 1). |
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| PARP; PPOL; ARTD1; Adprp; Adprt1; msPARP; parp-1; sPARP-1; 5830444G22Rik |
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| Human |
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| 11545 |
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| Q921K2 |
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| DEVDGTDEVA KKKSKKGKDK DSSKLEKALK AQNELIWNIK DELKKACSTN DLKELLIFNQ QQVPSGESAI LDRVADGMAF GALLPCKECS GQLVFKSDAY |
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| A synthetic peptide corresponding to a sequence within amino acids 211-310 of Mouse Cleaved PARP (Asp214) (NP_031441.2). |
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| Rabbit |
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| Affinity Purified Monoclonal IgG |
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| WB, ELISA |
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| Mouse |
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| 113kDa |
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| WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 _g/mL. Please optimize the concentration based on your specific assay requirements. |
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Reconstituted antibodies stable at -80C for 12 months, 4C for 1 week. Avoid repeated freeze-thaw cycles.
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Shipped at ambient temperature.
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Centrifuge tubes before opening. Dissolve the lyophilized antibodies in distilled water. 5-10% glycerol is recommended.
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| .jpg )
Western blot analysis of lysates from NIH/3T3 cells using Cleaved PARP (Asp214) Rabbit mAb (LTA24810) at 1:1000 dilution incubated overnight at 4_. NIH/3T3 cells were treated by staurosporine(1 _M) for 3 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LT014) at 1:10000 dilution.|Lysates/proteins: 30 _g per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit (LT00020).| Exposure time: 90s. |
