Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein (LTP10687)

Product Name
Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein (LTP10687)
Catalog Number
LTP10687
Quantity
100ug
Price
$563 In stock
Background
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3? chain, a CD3? chain, and two CD3?u chains.
Description
Recombinant Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal. It contains Asp22-Thr48.
Target
CD3E/CD3 epsilon 1-27
Species
Cynomolgus
Gene/Protein ID
Q95LI5.2
Exact Sequence
Asp22-Thr48
Molecular Weight
The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Cynomolgus CD3E 1-27 peptide, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 37.2ng/ml determined by ELISA. See testing image for detail.
Tag
C-hFc-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein (LTP10687)
  • 5 Units in Stock

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