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| Cynomolgus IgE Protein (LTP10378) |
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| LTP10378 |
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| 100ug |
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$563 In stock |
| Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, Fc?uRI and CD23 (Fc?uRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule. |
| Recombinant Cynomolgus IgE Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Cys208-Lys429. |
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| IgE |
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| Cynomolgus |
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| G8F4W7 |
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| Cys208-Lys429 |
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| The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Cynomolgus IgE, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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