Catalog #: LT483449
Sequence: {DABCYL}-KTSAVLQSGFRKM-E(EDANS)
Molecular Formula: C95H141N25O24S2
Molecular Weight: 2081.39
Quantity: 5mg
Purity: >95%
CAS Registry #: 730985-86-1
Dabcyl-KTSAVLQSGFRKME-Edans: Dabcyl-KTSAVLQSGFRKME-Edans, TFA, Dabcyl-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-Met-Glu-Edans, is a fluorogenic peptide. Dabcyl-KTSAVLQSGFRKME-Edans TFA is used as the substrate to measure the enzymatic activities of protease forms.
The Dabcyl-KTSAVLQSGFRKME-Edans TFA fluorogenic peptide is used as the substrate and the enhanced fluorescence due to cleavage of this substrate catalyzed by the protease is monitored at 538 nm with an excitation wavelength of 355 nm using a fluorescence plate reader. Sensitive internally quenched fluorogenic (FRET) substrate for SARS main protease with a Km value of 17 uM and a kcat value of 1.9 s-1.
Synonym(s): DABCYL-KTSAVLQSGFRKME-EDANS, DABCYL-Lys-HCoV-SARS Replicase Polyprotein 1ab (3235-3246)-Glu-EDANS trifluoroacetate salt, covid, covid-19, covid19, coronavirus, SARS-CoV-2 3CL Protease Substrate
Reference: ...3CLpro substrate {Dabcyl}-KTSAVLQSGFRKM-{Glu(Edans)} was purchased from LifeTein (purity >95%). .. https://doi.org/10.1016/j.ejmech.2023.115186
