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| DNMT3B Rabbit mAb |
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| LTA24731 |
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| 100ul |
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$750
In stock
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| CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. |
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| ICF; ICF1; FSHD4; M.HsaIIIB |
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| 1789,sc-10236,sc-10235,sc-130740,sc-20704,DNMT3B,ICF,ICF1,M.HsaIIIB,DNA methyltransferase 3 beta,DNA (cytosine-5)-methyltransferase 3B,DNA (cytosine-5-)-methyltransferase 3 beta,DNA MTase HsaIIIB,DNA cytosine-5--methyltransferase 3 beta,DNA methyltransferase HsaIIIB,Q9UBC3,DNA Methyltransferase 3 Beta,DNA (Cytosine-5-)-Methyltransferase 3 Beta,DNA Methyltransferase HsaIIIB,DNA Cytosine-5--Methyltransferase 3 Beta,DNA (Cytosine-5)-Methyltransferase 3B,EC 2.1.1.37,Dnmt3b |
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| Human |
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| 1789 |
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| Q9UBC3 |
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| MKGDTRHLNGEEDAGGREDSILVNGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRETRTRSESPAVRTRNNNSVSSRERHRPSPRSTRGRQGRNHVDESPVEFPATRSLRRRATASAGTPWPSPPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQDSQQGGMESPQVEADSGDGDSSEYQDGKEFGIGDLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRWVQWFGDGKFSEVSADKLVALGLFSQHFNLATFNKLVSYRKAMYHALEKARVRAGKTFPSSPGDSLEDQLKPMLEWAHGGFKPTGIEGLKPNNTQPVVNKSKVRRAGSRKLESRKYENKTRRRTADDSATSDYCPAPKRLKTNCYNNGKDR |
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| Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human DNMT3B (NP_008823.1). |
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| Rabbit |
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| Affinity Purified Monoclonal IgG |
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| WB, ELISA |
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| Mouse |
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| 96kDa |
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| WB,1:2500 - 1:5000|ELISA,Recommended starting concentration is 1 _g/mL. Please optimize the concentration based on your specific assay requirements. |
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Reconstituted antibodies stable at -80C for 12 months, 4C for 1 week. Avoid repeated freeze-thaw cycles.
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Shipped at ambient temperature.
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Centrifuge tubes before opening. Dissolve the lyophilized antibodies in distilled water. 5-10% glycerol is recommended.
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| .jpg )
Western blot analysis of various lysates using DNMT3B Rabbit mAb (LTA24731) at 1:2800 dilution incubated overnight at 4_.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LT014) at 1:10000 dilution.|Lysates/proteins: 25 _g per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (LT00020).|Exposure time: 90s. |
