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Description:
Fmoc-PNA-U-OH is a specialized monomer utilized in the synthesis of Peptide Nucleic Acids (PNAs), which are synthetic analogs of DNA with a neutral peptide-like backbone. This monomer is designed for compatibility with automated DNA synthesizers and can be adapted for use with other synthetic chemistry instruments, including microwave-assisted peptide synthesizers.
Key Features:
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High-Affinity Binding: PNAs, including those synthesized with this monomer, exhibit higher binding affinity to DNA strands than DNA itself, allowing for effective use in shorter oligomer lengths of 12-15 units.
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Aggregation Control: It's recommended to limit purine content, especially G-rich sequences, to prevent aggregation. Incorporating lysine or multiple AEAA linkers can improve solubility and handling.
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Labelling Flexibility: Fmoc-PNA-U-OH allows for the labeling of PNA with various groups like fluorescein and biotin. Labels can be attached either to the resin before cleavage or in solution after cleavage, depending on their acid sensitivity.
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Solid Support Compatibility: Suitable for use with solid supports like PAL-PEG-PS and XAL-PEG-PS, which are cleavable in acid and compatible with Fmoc chemistry.
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Optimized Synthesis Protocol: The synthesis involves a repetitive cycle of deblocking, activation/coupling, and capping, typically conducted on a 2umol scale.
Applications:
- Synthesis of short, high-affinity PNAs for DNA binding studies.
- Research in molecular biology, especially in areas requiring specific DNA sequence recognition and binding.
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