Human 4-1BB/TNFRSF9 Protein (LTP10677)

Product Name
Human 4-1BB/TNFRSF9 Protein (LTP10677)
Catalog Number
LTP10677
Quantity
100ug
Price
$406 In stock
Background
4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.
Description
Recombinant Human 4-1BB/TNFRSF9 Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal. It contains Leu24-Gln186.
Target
4-1BB/TNFRSF9
Species
Human
Gene/Protein ID
Q07011
Exact Sequence
Leu24-Gln186
Molecular Weight
The protein has a predicted MW of 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human 4-1BB, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand, hFc Tag with the EC50 of 0.15ug/ml determined by ELISA. See testing image for detail.
Tag
C-hFc-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human 4-1BB/TNFRSF9 Protein (LTP10677)
  • 5 Units in Stock

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