| Human AFP (HLA-A*02:01) Protein (LTP10216) |
| LTP10216 |
| 100ug |
|
$406 In stock |
| Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response. |
| Recombinant Human AFP (HLA-A*02:01) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide. |
| AFP (HLA-A*02:01) |
| Human |
| A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI |
| Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and FMNKFIYEI peptide |
| The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on the Tris-Bis PAGE result. |
| Immobilized Human AFP (HLA-A*02:01) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-AFP (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail. |
| C-His-Avi |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| ![Human AFP (HLA-A*02:01) Protein (LTP10216)](/peptide-product/images/protein-products/LTP10216/20220324141337.png) |