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| Human B7-H3 (4Ig) /B7-H3b Protein (LTP10613) |
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| LTP10613 |
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| 100ug |
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$406 In stock |
| B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig?B7?H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. |
| Recombinant Human B7-H3 (4Ig)/B7-H3b Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Gly27-Thr461. |
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| B7-H3 (4Ig)/B7-H3b |
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| Human |
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| Q5ZPR3-1 |
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| Gly27-Thr461 |
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| The protein has a predicted MW of 73.4 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human B7-H3 (4Ig) , hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA. See testing image for detail. |
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| C-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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