| Human B7-H3/CD276 Protein (LTP10667) |
| LTP10667 |
| 100ug |
|
$360 In stock |
| B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig?B7?H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. |
| Recombinant Human B7-H3/CD276 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Leu29-Pro245. |
| B7-H3/CD276 |
| Human |
| Q5ZPR3-2 |
| Leu29-Pro245 |
| The protein has a predicted MW of 50.1 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on the Tris-Bis PAGE result. |
| Immobilized Human B7-H3, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 42.2ng/ml determined by ELISA. See testing image for detail. |
| C-hFc |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| ![Human B7-H3/CD276 Protein (LTP10667)](/peptide-product/images/protein-products/LTP10667/20220120152500.png) |