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| Human B7-H5/Gi24/VISTA Protein (LTP10624) |
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| LTP10624 |
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| 100ug |
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$316 In stock |
| B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. |
| Recombinant Human B7-H5/Gi24/VISTA Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Phe33-Ala194. |
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| B7-H5/Gi24/VISTA |
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| Human |
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| Q9H7M9 |
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| Phe33-Ala194 |
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| The protein has a predicted MW of 44.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human B7-H5, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). The affinity constant of 14.44 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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