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| Human BAFF/TNFSF13B/CD257 Protein (LTP10736) |
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| LTP10736 |
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| 100ug |
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$473 In stock |
| B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. |
| Recombinant Human BAFF/TNFSF13B/CD257 Protein is expressed from Expi293 with hFc tag at the N-terminal. It contains Ala134-Leu285. |
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| BAFF/TNFSF13B/CD257 |
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| Human |
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| Q9Y275-1 |
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| Ala134-Leu285 |
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| The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human BCMA Trimer, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 17ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| N-monomeric hFc |
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| Expi293 |
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| > 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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