Human BTN3A1/CD277 Protein (LTP10596)

Product Name
Human BTN3A1/CD277 Protein (LTP10596)
Catalog Number
LTP10596
Quantity
100ug
Price
$406 In stock
Background
The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human V?9/V?2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
Description
Recombinant Human BTN3A1/CD277 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln30-Gly254.
Target
BTN3A1/CD277
Species
Human
Gene/Protein ID
O00481-1
Exact Sequence
Gln30-Gly254
Molecular Weight
The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human BTN3A1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. See testing image for detail.
Tag
C-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human BTN3A1/CD277 Protein (LTP10596)
  • 5 Units in Stock

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