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| Human CA125/MUC16 Protein (LTP10876) |
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| LTP10876 |
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| 100ug |
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$473 In stock |
| MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN. |
| Recombinant Human CA125/MUC16 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly12660-Met12923. |
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| CA125/MUC16 |
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| Human |
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| Q8WXI7 |
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| Gly12660-Met12923 |
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| The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 50-80 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CA125, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 1.1ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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