Human CD117 Protein (LTP10592)

Product Name
Human CD117 Protein (LTP10592)
Catalog Number
LTP10592
Quantity
100ug
Price
$360 In stock
Background
The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.
Description
Recombinant Human CD117 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln26-Thr516.
Target
CD177
Species
Human
Gene/Protein ID
P10721-2
Exact Sequence
Gln26-Thr516
Molecular Weight
The protein has a predicted MW of 56.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human CD117, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Tag
C-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human CD117 Protein (LTP10592)
  • 5 Units in Stock

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