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| Human CD19 Protein (LTP10852) |
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| LTP10852 |
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| 100ug |
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$563 In stock |
| CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses.Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores. |
| Recombinant Human CD19 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Pro20-Lys291. |
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| CD19 |
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| Human |
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| P15391-1 |
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| Pro20-Lys291 |
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| The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 50-60 KDa based on Bis-Tris PAGE result. |
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| Immobilized Human CD19, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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