Human CD200 R1/CRTR2 Protein (LTP11000)

Product Name
Human CD200 R1/CRTR2 Protein (LTP11000)
Catalog Number
LTP11000
Quantity
100ug
Price
$406 In stock
Background
CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.
Description
Recombinant Human CD200 R1/CRTR2 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala27-Leu266.
Target
CD200 R1/CRTR2
Species
Human
Gene/Protein ID
AAQ19772
Exact Sequence
Ala27-Leu266
Molecular Weight
The protein has a predicted MW of 29.7 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human CD200 R1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human CD200 R1/CRTR2 Protein (LTP11000)
  • 5 Units in Stock

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