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| Human CD30 Ligand/TNFSF8 Protein (LTP10808) |
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| LTP10808 |
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| 100ug |
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$473 In stock |
| CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. |
| Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from Expi293 with mFc (IgG2a) tag at the N-terminal. It contains Gln63-Asp234. |
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| CD30 Ligand/TNFSF8 |
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| Human |
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| P32971-1 |
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| Gln63-Asp234 |
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| The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CD30, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc (IgG2a) Tag with the EC50 of 31.9ng/ml determined by ELISA. See testing image for detail. |
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| N-mFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC; |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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