Human CEACAM-5/CD66e Protein (LTP11013)

Product Name
Human CEACAM-5/CD66e Protein (LTP11013)
Catalog Number
LTP11013
Quantity
100ug
Price
$339 In stock
Background
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated.
Description
Recombinant Human CEACAM-5/CD66e Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Lys35-Ala685.
Target
CEACAM-5/CD66e
Species
Human
Gene/Protein ID
Q8N4D0
Exact Sequence
Lys35-Ala685
Molecular Weight
The protein has a predicted MW of 74.2 kDa. Due to glycosylation, the protein migrates to 115-150 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human CEACAM-5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-5 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. The affinity constant of 0.12nM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human CEACAM-5/CD66e Protein (LTP11013)
  • 5 Units in Stock

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