Human EpCAM/TROP1 Protein (LTP10593)

Product Name
Human EpCAM/TROP1 Protein (LTP10593)
Catalog Number
LTP10593
Quantity
100ug
Price
$293 In stock
Background
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP?1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF?like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Description
Recombinant Human EpCAM/TROP1 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Gln24-Lys245.
Target
EpCAM/TROP1
Species
Human
Gene/Protein ID
P16422
Exact Sequence
Gln24-Lys245
Molecular Weight
The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human EpCAM, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 18.9ng/ml determined by ELISA. See testing image for detail.
Tag
C-hFc
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human EpCAM/TROP1 Protein (LTP10593)
  • 5 Units in Stock

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