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| Human Fas Ligand/TNFSF6 Protein (LTP11124) |
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| LTP11124 |
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| 100ug |
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$630 In stock |
| Fas ligand (FasL, CD95L) is a 40-kDa type II transmembrane protein that binds to Fas (CD95) receptors and promotes programmed cell death. Fas receptors are expressed at higher levels in many tumors than in normal cells.Somewhat paradoxically, elimination of Fas or FasL from tumors also leads to death induced by CD95 receptor/ligand elimination (DICE). |
| Recombinant Human Fas Ligand/TNFSF6 Protein is expressed from Expi293 with His tag at the N-terminal. It contains Pro134-Leu281. |
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| Fas Ligand/TNFSF6 |
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| Human |
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| AAH17502.1 |
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| Pro134-Leu281 |
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| The protein has a predicted MW of 18 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human Fas Ligand, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| N-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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