Human Fc gamma RIIB/CD32b Protein (LTP10807)

Product Name
Human Fc gamma RIIB/CD32b Protein (LTP10807)
Catalog Number
LTP10807
Quantity
100ug
Price
$360 In stock
Background
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Description
Recombinant Human Fc gamma RIIB/CD32b Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala46-Pro217.
Target
Fc gamma RIIB/CD32b
Species
Human
Gene/Protein ID
P31994-1
Exact Sequence
Ala46-Pro217
Molecular Weight
The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on the Tris-Bis PAGE result.
Activity
The affinity constant of 3.46 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human Fc gamma RIIB/CD32b Protein (LTP10807)
  • 5 Units in Stock

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