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| Human FcRn Protein (LTP10232) |
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| LTP10232 |
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| 100ug |
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$485 In stock |
| The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer. |
| Recombinant Human FcRn Protein is expressed from Expi293 with His tag at the C-terminal. It contains Ala24-Ser297(FCGRT) & Ile21-Met119(B2M). |
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| FcRn |
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| Human |
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| P55899-1(FCGRT)&P61769-1(B2M) |
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| Ala24-Ser297(FCGRT) & Ile21-Met119(B2M) |
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| The protein has a predicted MW of 31.5 kDa (FCGRT) & 11.7 kDa (B2M). Due to glycosylation, the protein migrates to 35-40 kDa (FCGRT) & 13-14 kDa (B2M) based on the Tris-Bis PAGE result. |
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| Immobilized Human FcRn, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.40 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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