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| Human Glypican 1/GPC1 Protein (LTP10545) |
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| LTP10545 |
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| 100ug |
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$473 In stock |
| CAR-T cells targeting glypican-1 (GPC1)-specific human and murine CAR-T cells generated from our original anti-human/mouse GPC1 antibody showed strong antitumor effects in xenogeneic and syngeneic mouse models, respectively. Importantly, the murine CAR-T cells enhanced endogenous T cell responses against a non-GPC1 tumor antigen through the mechanism of antigen-spreading and showed synergistic antitumor effects with anti-PD-1 antibody without any adverse effects in syngeneic models. |
| Recombinant Human Glypican 1/GPC1 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Asp24-Ser530. |
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| Glypican 1/GPC1 |
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| Human |
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| P35052-1 |
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| Asp24-Ser530 |
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| The protein has a predicted MW of 57.1 kDa. Due to glycosylation, the protein migrates to 70-135 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human Glypican 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Glypican 1 Antibody, hFc Tag with the EC50 of 21.9ng/ml determined by ELISA (QC Test). See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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