Human Her2/ErbB2 Protein (LTP10577)

Product Name
Human Her2/ErbB2 Protein (LTP10577)
Catalog Number
LTP10577
Quantity
100ug
Price
$496 In stock
Background
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane.
Description
Recombinant Human Her2/ErbB2 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Thr23-Thr652.
Target
Her2/ErbB2
Species
Human
Gene/Protein ID
P04626-1
Exact Sequence
Thr23-Thr652
Molecular Weight
The protein has a predicted MW of 96.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human Her2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Her2 Antibody , hFc Tag with the EC50 of 0.26ug/ml determined by ELISA. See testing image for detail.
Tag
C-hFc
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human Her2/ErbB2 Protein (LTP10577)
  • 5 Units in Stock

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