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| Human IL-13 Protein (LTP10448) |
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| LTP10448 |
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| 100ug |
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$360 In stock |
| Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity). |
| Recombinant Human IL-13 Protein is expressed from Expi293 with hFc tag at the N-terminal. It contains Gly21-Asn132. |
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| IL-13 |
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| Human |
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| AAK53823 |
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| Gly21-Asn132 |
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| The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human IL-13, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-13Ra1, His Tag with the EC50 of 0.83ug/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| N-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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