|
| Human IL-5 Protein (LTP10535) |
|
| LTP10535 |
|
| 100ug |
|
|
$496 In stock |
| IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(R?) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA). |
| Recombinant Human IL-5 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Ile20-Ser134. |
|
| IL-5 |
|
| Human |
|
| P05113 |
|
| Ile20-Ser134 |
|
| The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Human IL-5 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 21.3ng/ml determined by ELISA (QC Test). The affinity constant of 4.9nM as determined in SPR assay (Biacore T200). See testing image for detail. |
|
| N-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
