| Human IL-7 Protein (LTP11015) |
| LTP11015 |
| 100ug |
|
$316 In stock |
| Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7R? or ? c chain, the two subunits that constitute the functional IL7 receptor. |
| Recombinant Human IL-7 Protein is expressed from E.coli without tag. It contains Asp26-His177. |
| IL-7 |
| Human |
| P13232 |
| Asp26-His177 |
| The protein has a predicted MW of 17.5 kDa same as Tris-Bis PAGE result. |
| Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 55.1ng/ml determined by ELISA. The affinity constant of 22.66 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| No Tag |
| E.coli |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in 25mM PB, 250mM NaCl (pH 6.8). Normally 8% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 25mM PB, 250mM NaCl (pH 6.8). |
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