| Human Latent TGF beta 1/TGFB1 Protein (LTP10931) |
| LTP10931 |
| 100ug |
|
$609 In stock |
| Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin?like convertase processes the proprotein to generate an N?terminal 249 aa latency?associated peptide (LAP) and a C?terminal 112 aa mature TGF? beta 1. Disulfide?linked homodimers of LAP and TGF? beta 1 remain non?covalently associated after secretion, forming the small latent TGF? beta 1 complex. |
| Recombinant Human Latent TGF beta 1/TGFB1 Protein is expressed from Expi293 with His tag at the N-terminal. It contains Leu30-Ser390(C33S). |
| Latent TGF beta 1/TGFB1 |
| Human |
| P01137 |
| Leu30-Ser390(C33S) |
| The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa(LAP) / 13-15 kDa(Mature TGF Beta 1) kDa based on the Tris-Bis PAGE result. |
| Immobilized Human Latent TGF beta 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 86.1ng/ml determined by ELISA. See testing image for detail. |
| N-His |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| ![Human Latent TGF beta 1/TGFB1 Protein (LTP10931)](/peptide-product/images/protein-products/LTP10931/20211220170453.png) |