Human MAGE-A4 (HLA-A*02:01) Protein (LTP10827)

Product Name
Human MAGE-A4 (HLA-A*02:01) Protein (LTP10827)
Catalog Number
LTP10827
Quantity
100ug
Price
$406 In stock
Background
Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and GVYDGREHTV peptide of  the MAGE-A4.
Description
Recombinant Human MAGE-A4 (HLA-A*02:01) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and GVYDGREHTV peptide.
Target
MAGE-A4 (HLA-A*02:01)
Species
Human
Gene/Protein ID
A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV
Exact Sequence
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and GVYDGREHTV peptide
Molecular Weight
The protein has a predicted MW of 50.5 kDa. Due to glycosylation,the protein migrates to 52-60 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human MAGE-A4 (HLA-A*02:01) , His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human MAGE-A4 (HLA-A*02:01) Protein (LTP10827)
  • 5 Units in Stock

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