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| Human Mature TGF beta 1 Protein (LTP11029) |
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| LTP11029 |
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| 100ug |
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$406 In stock |
| TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition.Transforming growth factor beta-1 is multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. |
| Recombinant Human Mature TGF beta 1 Protein is expressed from Expi293 without tag. It contains Ala279-Ser390. |
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| Mature TGF beta 1 |
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| Human |
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| P01137 |
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| Ala279-Ser390 |
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| The protein has a predicted MW of 13.2 kDa (monomer). Due to glycosylation, the protein migrates to 14-15 kDa (monomer) under reduced (R) condition and 26-30 kDa (dimer) under Non reducing (N) condition based on the Tris-Bis PAGE result. |
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| Immobilized Human Mature TGF beta 1, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 8ng/ml determined by ELISA. The affinity constant of 0.35 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| No Tag |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in 50mM Glycine 150mM NaCl (pH 2.5). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM Glycine 150mM NaCl (pH 2.5). |
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