Human Mature TGF beta 1 Protein (LTP11029)

Product Name
Human Mature TGF beta 1 Protein (LTP11029)
Catalog Number
LTP11029
Quantity
100ug
Price
$406 In stock
Background
TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition.Transforming growth factor beta-1 is multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration.
Description
Recombinant Human Mature TGF beta 1 Protein is expressed from Expi293 without tag. It contains Ala279-Ser390.
Target
Mature TGF beta 1
Species
Human
Gene/Protein ID
P01137
Exact Sequence
Ala279-Ser390
Molecular Weight
The protein has a predicted MW of 13.2 kDa (monomer). Due to glycosylation, the protein migrates to 14-15 kDa (monomer) under reduced (R) condition and 26-30 kDa (dimer) under Non reducing (N) condition based on the Tris-Bis PAGE result.
Activity
Immobilized Human Mature TGF beta 1, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 8ng/ml determined by ELISA. The affinity constant of 0.35 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
No Tag
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in 50mM Glycine 150mM NaCl (pH 2.5). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM Glycine 150mM NaCl (pH 2.5).

Image
Human Mature TGF beta 1 Protein (LTP11029)
  • 5 Units in Stock

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