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| Human M-CSF R/CSF1R/CD115 Protein (LTP10566) |
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| LTP10566 |
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| 100ug |
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$360 In stock |
| CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. |
| Recombinant Human M-CSF R/CSF1R/CD115 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Ile20-Glu512. |
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| M-CSF R/CSF1R/CD115 |
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| Human |
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| P07333-1 |
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| Ile20-Glu512 |
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| The protein has a predicted MW of 81.3 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human M-CSF, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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