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| Human M-CSF/CSF-1 Protein (LTP10919) |
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| LTP10919 |
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| 100ug |
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$676 In stock |
| CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. |
| Recombinant Human M-CSF/CSF-1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu33-Asn190. |
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| M-CSF/CSF-1 |
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| Human |
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| P09603-1 |
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| Glu33-Asn190 |
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| The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 28-34 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human M-CSF, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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