Human MMP-9 Protein (LTP11008)

Product Name
Human MMP-9 Protein (LTP11008)
Catalog Number
LTP11008
Quantity
100ug
Price
$473 In stock
Background
Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.
Description
Recombinant Human MMP-9 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. The protein needs to be activated by APMA to have hydrolytic activity. It contains Ala20-Asp707.
Target
MMP-9
Species
Human
Gene/Protein ID
P14780
Exact Sequence
Ala20-Asp707
Molecular Weight
The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human MMP-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human MMP-9 Protein (LTP11008)
  • 5 Units in Stock

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