Human Nectin-1/PVRL1/CD111 Protein (LTP10886)

Product Name
Human Nectin-1/PVRL1/CD111 Protein (LTP10886)
Catalog Number
LTP10886
Quantity
100ug
Price
$360 In stock
Background
Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity.
Description
Recombinant Human Nectin-1/PVRL1/CD111 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln31-Thr334.
Target
Nectin-1/PVRL1/CD111
Species
Human
Gene/Protein ID
Q15223
Exact Sequence
Gln31-Thr334
Molecular Weight
The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on the Tris-Bis PAGE result.
Activity
The affinity constant of 28.13 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human Nectin-1/PVRL1/CD111 Protein (LTP10886)
  • 5 Units in Stock

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