|
| Human Nectin-3/CD113 Protein (LTP10911) |
|
| LTP10911 |
|
| 100ug |
|
|
$473 In stock |
| The Nectin family has at least four members (Nectin-1?4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids. |
| Recombinant Human Nectin-3/CD113 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly58-Asp400. |
|
| Nectin-3/CD113 |
|
| Human |
|
| Q9NQS3 |
|
| Gly58-Asp400 |
|
| The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on the Tris-Bis PAGE result. |
|
| The affinity constant of 0.204 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
|
| C-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
