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| Human NKG2A&CD94 Protein (LTP10653) |
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| LTP10653 |
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| 100ug |
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$473 In stock |
| The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies. |
| Recombinant Human NKG2A&CD94 Protein is expressed from Expi293 with hFc tag and Flag tag at the N-terminal. It contains Arg100-Ala219(NKG2A)&Ser34-Ile179(CD94). |
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| NKG2A&CD94 |
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| Human |
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| P26715-1(NKG2A)&Q13241-1(CD94) |
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| Arg100-Ala219(NKG2A) & Ser34-Ile179(CD94) |
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| The protein has a predicted MW of 17.9 kDa (CD94)&41.1 kDa (NKG2A). Due to glycosylation, the protein migrates to 50-70 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human NKG2A&CD94, hFc tag at 1ug/ml (100ul/Well) on Fc Antibody (5ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Ab., hFc Tag with the EC50 of 15.1ng/ml determined by ELISA. See testing image for detail. |
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| N-hFc-Flag |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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