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| Human NKG2D/CD314 Protein (LTP10817) |
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| LTP10817 |
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| 100ug |
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$406 In stock |
| NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma ? T cells, NK cells and NKT cells. |
| Recombinant Human NKG2D/CD314 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Phe78-Val216. |
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| NKG2D/CD314 |
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| Human |
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| P26718 |
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| Phe78-Val216 |
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| The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-38 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| N-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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