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| Human PDGF R beta/CD140b Protein (LTP10940) |
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| LTP10940 |
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| 100ug |
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$339 In stock |
| PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. |
| Recombinant Human PDGF R beta/CD140b Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu33-Phe530. |
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| PDGF R beta/CD140b |
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| Human |
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| P09619 |
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| Leu33-Phe530 |
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| The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 78-115 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human PDGF R beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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