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| Human RGMa Protein (LTP10981) |
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| LTP10981 |
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| 100ug |
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$406 In stock |
| Repulsive guidance molecule (RGM) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that has diverse functions in the developing and pathological central nervous system (CNS). The binding of RGM to its receptor neogenin regulates axon guidance, neuronal differentiation, and survival during the development of the CNS. RGMa induces T cell activation in experimental autoimmune encephalomyelitis (EAE), which is the animal model of multiple sclerosis (MS). RGM is expressed in pathogenic Th17 cells and induces neurodegeneration by binding to neogenin. |
| Recombinant Human RGMa Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Cys48-Gly422. |
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| RGMa |
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| Human |
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| Q96B86-1 |
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| Cys48-Gly422 |
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| The protein has a predicted MW of 44.5 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.45ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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