| Human RNF43 Protein (LTP10350) |
| LTP10350 |
| 100ug |
|
$406 In stock |
| RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/?-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation. |
| Recombinant Human RNF43 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Gly24-Tyr197. |
| RNF34 |
| Human |
| Q68DV7 |
| Gly24-Tyr197 |
| The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on the Tris-Bis PAGE result. |
| Immobilized Human R-Spondin 3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA (QC Test). See testing image for detail. |
| C-hFc |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| ![Human RNF43 Protein (LTP10350)](/peptide-product/images/protein-products/LTP10350/20221117181012.png) |