|
| Human Siglec-10 Protein (LTP10829) |
|
| LTP10829 |
|
| 100ug |
|
|
$473 In stock |
| Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. |
| Recombinant Human Siglec-10 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Met17-Thr546. |
|
| Siglec-10 |
|
| Human |
|
| Q96LC7-1 |
|
| Met17-Thr546 |
|
| The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-79 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Human Siglec-10, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Ab., hFc Tag with the EC50 of 7.4ng/ml determined by ELISA. The affinity constant of 15.9uM as determined in SPR assay (Biacore T200). See testing image for detail. |
|
| C-His |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in 20mM PB, 150mM NaCl, 25mM L-Arginine (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
