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| Human Siglec-3/CD33 Protein (LTP10870) |
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| LTP10870 |
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| 100ug |
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$406 In stock |
| Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. |
| Recombinant Human Siglec-3/CD33 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Asp18-His259. |
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| Siglec-3/CD33 |
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| Human |
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| P20138-1 |
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| Asp18-His259 |
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| The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human Siglec-3, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. See testing image for detail. |
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| C-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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